You are asked to cite AmplifX in your publications:
Designing primers using amplifx registration#
Registration will allow users to be kept informed for new updates and important informations about this program as soon as they are available. Users are strongly invited to register themselves with the online form accessible by the help menu and send any bug repport. The author couldn't be responsible for any bad working or lost of data. This program is provided free for the scientific community ”as this”. J Mol Diagn 2010 and personnal unpublished results) Legal informations Since version 2, AmplifX allows to design primers and run PCR for dCAPS analysis: it means that the new algorithms allow mismatches in the 3' end of primers that work in real PCR (Stadhouders et al. Testing primers in in silico PCR: Do not try to remember if you have some primers that can be used for your new project: just test all of your collection in AmplifX and predict PCR efficiency, reaction parameters, amplicon size, etc.ĭesign primers: AmplifX can design primers by the way of an intuitive interface: just select the region of interest into you target sequence and let AmplifX choose the best primer sequences or play with intuitive parameters to make more challenging design. You will be able to add some personnal informations (ID, descriptive name, comments, color coded category, owner, etc.) You will eventually be able to sort, filter, retrieve, share with your colleagues and of course test them in silico (see below). expected efficiency in PCR reaction), etc.
Managing primers: keep all the informations for your hundreds or thousands of primers into a single database and retrieve the tubes in the freezer in just a minute! For each primer, AmplifX will calculate some important features: TM, "Quality" (i.e. AmplifX is a program dedicated to Polymerase Chain Reaction (PCR) experiments.
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